How can I disable a signature file that's being attached automatically to my Microsoft Exchange e-mail?
That depends on what is generating it. If it's your Outlook or Exchange client, then go into Tools | Options and you can turn it off in there. If its coming from a server or mail gateway in your organization (for example, a company disclaimer), then you'll need to turn it off at that point of origin.
Dig deeper on Microsoft Exchange Server Mailbox Management
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